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門派: 殺豬拔毛派(萌主)
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來自: 萌萌異境 
發文: 7334 篇
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在線: 733 時 35 分 50 秒
註冊: 2001/12/06
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 Message  查看  搜尋  通訊錄  複製  引用  回覆 | [第 12 樓] |
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Secondary metabolites in in vitro cultured plants of the genus Drosera
abstract
Extracts from plantlets of different species of the genus Drosera, grown as in vitro cultures, were evaluated for the level of phenolic secondary metabolites from the group of naphthoquinones and flavonols. The profiles of natural products in the extracts obtained from different species were monitored by HPLC with UV detection at 260 and 330 nm. On the basis of the data obtained, Drosera binata, the species with the highest amount of plumbagin, was selected for further studies. The most effective method of extraction of quinones was established and the composition of phenolic secondary metabolites in the tissues was determined. For the identification of phenolic compounds, HPLC-UV and HPLC-ESI/MS were applied.
source
Marczak L, Kawiak A, Lojkowska E, Stobiecki M. Phytochem Anal. 2005, 16: 143-149.
link
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15997845
標題
試管培養毛氈苔之二次代謝產物
摘要
我們研究在不同種類中，試管培養毛氈苔培植體之萃取物其源自於naphehoquinone和黃酮醇的酚類二次代謝物的含量。不同種類的毛氈苔萃取物之天然物圖譜是以高效液相層析儀以260和330 nm紫外光波段來獲得。基於所獲得資料，D. binata是所有分析的毛氈苔中含有最大量的礬松素，所以這種毛氈苔再用於下一步的研究。我們建立出最有效的醌類的萃取方法，並分析植物組織中的酚類二次代謝產物的成份。在鑑別酚類化合物上，我們使用紫外光高效液相層析儀及電灑法串聯式質譜高效液相層析儀。
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 2006/01/13 09:22am  IP: 已設定保密 | [本文共 1581 位元組]  |
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